Catalog No. : XBA0100

PDQeX prepGEM Bacteria (100)

PDQeX prepGEM Bacteria (100)

Description

·         For use with MicroGEM’s PDQex Nucleic Acid Extractor

·         Suitable for processing gram-positive bacteria, gram-negative bacteria, protozoa, archaea, colonies and liquid cultures, biofilm and mucosal samples, swabs, and metagenomic DNA including soil, stool and water Double-stranded DNA extracts suitable for whole genome sequencing, PCR, qPCR, targeted sequencing (amplicon sequencing) from metagenomic samples - 16SrDNA sequencing

·         MicroGEM’s PDQeX Bacteria Kit provides a simple automated approach to studying a wide range of microbial cultures. Using MicroGEM’s PDQeX Nucleic Acid Extractor, purified, double-stranded DNA is generated, making it the perfect solution when working with inhibitory samples (for example soil and stool) or performing NGS (Next Gen. sequencing). Every aspect of the MicroGEM method ensures the samples are processed quickly and gently. Minimal sample prep, closed tube processing, and gentle lysis ensure that cross contamination and DNA fragmentation are drastically reduced, making MicroGEM’s extraction process streamlined while preserving the integrity of low abundance species.

• Novel temperature controlled enzymatic lysis using a thermophilic proteinase in combination with mesophilic Lysozyme

• Rapid workflow- 1/4th the time taken by other methods

• Easily automated for high throughput • Closed system- no cross contamination and protection against pathogens

• Gentle lysis with no transfer steps- preserves DNA and improves community representation in metagenomic samples • Minimal handling and pipetting of samples- DNA is exceptionally intact and high molecular weight – perfect for long read nanopore sequencing

• Can be easily adapted to different sample types and workflows

See MicroGEM's PDQeX Nucleic Acid Extractor in action
ItemSize Application Price
PDQeX prepGEM Bacteria (100) $631.40

MicroGEM’s PDQeX Bacteria Kit provides a simple automated approach to studying a wide range of microbial cultures. Using MicroGEM’s PDQeX Nucleic Acid Extractor, purified, double-stranded DNA is generated, making it the perfect solution when working with inhibitory samples (for example soil and stool) or performing NGS (Next Gen. sequencing). 


Every aspect of the MicroGEM method ensures the samples are processed quickly and gently. Minimal sample prep, closed tube processing, and gentle lysis ensure that cross contamination and DNA fragmentation are drastically reduced, making MicroGEM’s extraction process streamlined while preserving the integrity of low abundance species.


Novel temperature controlled enzymatic lysis using a thermophilic proteinase in combination with mesophilic Lysozyme

Rapid workflow- 1/4th the time taken by other methods

Easily automated for high throughput

Closed system- no cross contamination and protection against pathogens

Gentle lysis with no transfer steps- preserves DNA and improves community representation in metagenomic samples

Minimal handling and pipetting of samples- DNA is exceptionally intact and high molecular weight – perfect for long read nanopore sequencing

Can be easily adapted to different sample types and workflows

Quick-Start Guide: PDQeX prepGEM Bacteria 

Rapid extraction of DNA suitable for NGS workflows from bacterial cultures using the PDQeX. BioTechniques. Vol.66, No. 5. 2019.
This study compared a conventional column purification method with the PDQeX Nucleic Acid Extractor for purifying DNA for whole genome sequencing. Based on six bacterial suspensions, the PDQeX performed much more rapidly – in under 30 minutes – while producing comparable high-quality extractions.

Inner Workings: Portable DNA sequencer helps farmers stymie devastating viruses. Proceedings of the National Academy of Sciences,116(9), 3351-3353. 2019. 

This study described the ability to screen Cassava crops for Cassava Mosaic Virus (CMV) using MicroGEM’s PDQeX for rapid DNA extraction.  The PDQeX was used as the enabling technology to complement DNA sequencing being conducted in the field in real-time.

$631.40