High-Pass Plus 0.75% Agarose cassette for 15kb and 30kb HP, includes reagents, 10pk
The High Pass Plus Cassette
The High Pass Plus™ gel cassette is the newest addition to the Pippin Family. As the name suggests, it is dedicated to BluePippin “High-Pass” DNA size selection which has been a go-to method for increasing the read lengths for long-read sequencing.
High Pass size selection removes smaller DNA fragments from a sheared genomic DNA (or sequencing library) while collecting the remaining larger fragments above a tightly controlled size threshold. This way, larger molecules can be presented to the detector or droplet, and better sequencing performance can be achieved.
Long-read sequencing sample prep has been improving overall, in terms of set-up time and workflow. An entirely new gel cassette dedicated to High Pass– the High Pass Plus is designed. It cuts the runtime in half and improve performance and yield.
Here’s a comparison between the standard BluePippin Cassette and the High Pass Plus:
The High Pass Plus cassette has a stocky separation column, so DNA has a shorter distance to travel. The wider column and increased taper provide higher resolution for a cleaner and more accurate size cut-off. The larger sample wells now allow a maximum load of 10ug (100% up from standard Blue Pippin cassettes). The size of the elution module and surface area of the filtration membrane, are increased, bringing about improved sample recovery and reproducibility.
Here’s what is accomplished, when compared to the current BluePippin standard:
• Half the run time
• Twice the loading capacity
• Better recovery and reproducibility
For Blue Pippin software requirement is v6.31/6.40 CD31, check documents section
|High-Pass Plus 0.75% Agarose cassette for 15kb and 30kb HP, includes reagents, 10pk||
Pre-Cast Agarose Gel Cassettes for DNA Size Selection
Gel cassettes for DNA selections are available in four agarose concentrations to provide a full range of size selection options between 100bp up to 50kb. For smaller fragment sizes, internal standards may be used for higher accuracy and increased sample capacity (from 4 to 5 samples/cassette). Higher molecular weight fragments require a dedicated lane to run a calibration marker. Run times are as little as 30 minutes, To resolve very large DNA fragments, pulsed-field runs may last several hours. View the BluePippin cassette reference chart to explore the DNA size selection options and run times.
Standard DNA Size Selection Cassettes
3% agarose, 100-250bp
2% agarose, 100-600bp
1.5% agarose, 250bp – 1.5kb
0.75% agarose, 1-50kb
High Pass Plus Cassettes
>15KB High Pass