Catalog No. : SAG-PIPD001

Pippin Prep DNA Size Selection System with complete accessory pack; monitor not included*

Pippin Prep DNA Size Selection System with complete accessory pack; monitor not included*

Description

DNA Size Selection for Next-Gen Sequencing (100bp – 1.5kb)


Short Read NGS Library Construction – For Inserts from 100bp to 1.5kb

The Pippin Prep facilitates library construction for the most popular NGS platforms and is recommended by Illumina and Ion Torrent for certain workflows. The platform features the ability to collect narrow and even fragment distributions, as well as the flexibility to collect wide ranges of fragments with minimal effort. Target sizes or ranges of sizes are entered in software, and fractions are collected in a buffer. Up to 5 samples per gel cassette may be run, with no possibility of cross-contamination.


Benefits of the Pippin Prep System

  • Saves time and effort compared to manual gel purification
  • Sample-to-sample reproducibility streamlines research
  • Higher sample quality improves downstream analysis

Key Downstream Technologies

  • Paired-end sequencing
  • emPCR
  • RNA-seq
  • ChIP-seq


ItemSize Application Price
Pippin Prep DNA Size Selection System with complete accessory pack; monitor not included*

1 Unit

Automated Electrophoresis Please Inquire


Pippin Prep Specs

Electrophoresis voltage
100V or 150V constant
Optical detection
535 nm excitation | 640 nm emission
Power requirements
100-240 VAC, 2.5 A, 50-60 Hz
Weight
15 lbs / 7 kg
Dimensions
7h x 11w x 21d (in) | 18h x 28w x 53 d (cm)

Cassette Kits

Kits include 10 ea. disposable pre-cast gel cassettes.  All necessary standards and reagents are included with the purchase.

Pre-Cast Agarose Gel Cassettes for DNA Size Selection

Gel cassettes for DNA selections are available in four agarose concentrations to provide a full range of size selection options between 100bp and 1.5kb, with limited capability up to 8 kb.  For smaller fragment sizes, internal standards may be used for higher accuracy and increased sample capacity (from 4 to 5 samples per cassette).  Run times are as little as 30 minutes. View the Pippin Prep cassette reference chart to explore the DNA size selection options and run times.

Gel cassette types and target ranges

  • 3% agarose, 100-250bp
  • 2% agarose, 100-600bp
  • 1.5% agarose, 250bp – 1.5kb

For these downstream technologies

  • miRNA isolation
  • DNA sequencing, RNA-seq, genotyping
  • DNA sequencing, ChIP-seq
  • Mate-pair sequencing




Paper

Sage Pippin: Say Goodbye to Manual Gels

Brochure

Pippin Family Brochure

Support

Pippin Prep Cassette Reference Chart 6_23 CD10

Pippin Prep User Manual v6.23 CD10

Pippin Prep Installation Guide

Pippin Prep IQ/OQ Document

Application Notes

Automated Size Selection of NEBNext Small RNA Libraries with the Sage Pippin Prep

"ChIP-Seq Libary Prep" Westerling, T., Center for Functional Cancer Epigenetics, Dana Farber Cancer Institute.

Case Studies

Case Study: "Tag Team: Scientists Use Pippin to Optimize Nextera Libraries", Zach Herbert, Dana Farber Core Facilities

Case Study: "New Take on RADseq Enables High-Throughput Variant Discovery", Hoekstra Lab, Harvard Univ.

Case Study: "Better Metagenomics Methods Offer Hope for Studies with Ultra-Low DNA", Sullivan Lab, Univ. Arizona

Case Study: "Precise Sizing and SMRT Sequencing Offer Unprecedented Read Length for Clinical Studies", Robert Sebra, Mt. Sinai

Cassette Guides

DNA Sequences of Internal Standards

Quick Guide CDF1510 Marker K

Quick Guide CSD1510 Marker A

Quick Guide CSD2010 Marker B

Quick Guide CSD3010 Marker C

Quick Guide CDF2010 Marker L

Quick Guide CDP3010 Marker P

Quick Guide CEF2010 Marker E

DNA Control Kit Guides

Guide CON1504, Control DNA, External Marker A

Guide CON3004, Control DNA, External Marker C

Guide CON2004, Control DNA, External Marker B, E, or M1

Guide CIS1504, Control DNA, Internal Standard K or R2

Guide CIS2004, Control DNA, Internal Standard L or V1

Guide CIS3004, Control DNA, Internal Standard F or Q2

Guide CON7504, Control DNA, External Marker D or S1


Software Support
Please Inquire

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