Catalog No. : MN-740300.50

NucleoSnap DNA Plasma (50)

NucleoSnap DNA Plasma (50)

Description

Features
Isolation of cell-free DNA from up to 10 mL blood plasma
• New column design (snap-off column) for quick processing of large sample volumes
• No need for carrier-RNA
• Optimized protocol for Cell-Free DNA BCT® ('Streck Tubes')



Applications
• Typical downstream applications: NGS, qPCR, ddPCR
• Highly efficient recovery of cell-free DNA from 'Liquid Biopsies'

* Kits to be used for research purposes only
** Cell-free DNA BCT® is a registered trademark of Streck, Inc.
ItemSize Application Price
NucleoSnap DNA Plasma (50)

50 Preps

Extraction $1,138.00


Includes

NucleoSnap® DNA Plasma Columns, Collection Tubes, buffers, Proteinase K


Principle/Procedure

NucleoSnap® DNA Plasma procedure


 

  

NucleoSnap® (Snap-off columns) principle

    

 

Further information regarding our newest NucleoSnap® technology


 Application data



Time-saving procedure

cfDNA can be isolated from 12 EDTA preserved samples with NucleoSnap® DNA Plasma in less than 50 min, whereas the procedure of competitor Q takes more than 80 min.


 

Efficient isolation of cfDNA from 5 mL human EDTA plasma

Isolation of cfDNA from EDTA plasma with the NucleoSnap® DNA Plasma kit and a vacuum-based kit from a competitor (Competitor Q). DNA yields were quantified by qPCR (Quantifiler® Human DNA Quantification Kit, ThermoFisher Scientific on an Applied Biosystems 7500 Real-Time PCR System, ThermoFisher Scientific).


Optimized protocol for cfDNA isolation from Cell-Free DNA BCT® (Streck)

cfDNA was isolated from three independent Cell-free DNA BCT® preserved samples with NucleoSnap® DNA Plasma (MN) and a vacuum-processed kit of a competitor (Q). The isolated cfDNA shows the typical peak at approx. 170 bp, indicated by an arrow. cfDNA was separated on a Bioanalyzer™ 2100 with a High Sensitivity DNA Kit (Agilent).



Isolation of cell-free DNA from urine with NucleoSnap® DNA Plasma kit*

Electropherogram of cfDNA isolated from 10 mL urine with the NucleoSnap® DNA Plasma kit. The cfDNA shows the typical size distribution (peak ~ 170 bp). cfDNA was separated on a Bioanalyzer™ 2100 with a High Sensitivity DNA Kit (Agilent).

* For processing volumes > 5 mL, additional lysis buffer has to be purchased.



Typical cfDNA yields from urine samples

cfDNA was isolated from 10 mL urine (three independent samples) with the NucleoSnap® DNA Plasma kit. DNA yields were quantified by qPCR (Quantifiler® Human DNA Quantification Kit, ThermoFisher Scientific on a Applied Biosystems 7500 Real-Time PCR Sys-tems, ThermoFisher Scientific).

$1,138.00

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