Catalog No. : MN-740120.50

NucleoSpin RNA Plant and Fungi (50)

NucleoSpin RNA Plant and Fungi (50)

Description

Features 
Universal kit for challenging plant and fungal samples
• Tailored protocols for diverse starting materials
• Convenient handling and efficient isolation of high integrity RNA
• NucleoSpin® RNA Plant and Fungi Filters for lysate clearing included



Applications*
• RNA isolation from plant and fungi
• Typical downstream applications: real-time RT-PCR, Northern blotting, primer extension, array technology,
   RNase protection assays

* Kits to be used for research purposes only
ItemSize Application Price
NucleoSpin RNA Plant and Fungi (50)

50 Preps

Extraction $460.90


Includes:
NucleoSpin® RNA Plant and Fungi Filter, NucleoSpin® Plant and Fungi Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 mL), buffers, RNase free water

Principle/Procedure
Plant and fungal material is diverse in terms of structure, cell wall composition, biochemical compounds and the types of organs. Common products for the RNA isolation from plant material usually work fine with samples such as Arabidopsis leaves, but fail to efficiently purify RNA from challenging samples. The NucleoSpin® RNA Plant and Fungi kit was developed to efficiently purify high integrity RNA from samples rich in secondary  metabolites, sugar, starch or other compounds that interfere with nucleic acid purification methods. The kit enables RNA isolation from diverse plant organs like seeds, roots, stems, needles and others. Of course, it can also be used for standard samples such as Arabidopsis leaves.

                   

Application data
 
Successfully tested sample types
Table 1 shows a selection of samples that were successfully tested with NucleoSpin® RNA Plant and Fungi.



   

NucleoSpin® RNA Plant and Fungi – RNA extraction data
The NucleoSpin® RNA Plant and Fungi kit enables efficient isolation of high integrity RNA from various sample types. After RNA isolation with NucleoSpin® RNA Plant and Fungi (MN) and two competitor products (S, Q), the RNA was analyzed and quantified with an Agilent Bioanalyzer.
(A) RNA isolation from 500 mg kiwi fruit
(B) RNA isolation from 50 mg potato tuber
(C) RNA isolation from 50 mg spruce needles


 

The NucleoSpin® RNA Plant and Fungi kit allows for extraction of high integrity RNA from fungal samples
The RNA was isolated from 3 independent samples of fungal fruiting body and fungal mycelium. Analysis of the RNA was performed with an Agilent Bioanalyzer
(D) RNA isolation from 50 mg Agaricus campestris fruiting body
(E) RNA isolation from 100 mg Botrytis cinerea mycelium

Summary of the RNA isolations from oil palm and avocado fruit flesh





RNA isolation from lipid rich fruit 
The NucleoSpin® RNA Plant and Fungi kit enables efficient isolation of RNA from difficult and badly stored samples that are rich in lipid content. After RNA isolation the RNA content and quality was analyzed and quantified with an Agilent Bioanalyzer.

  • NucleoSpin RNA Plant and Fungi (50)
$460.90

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