Catalog No. : MN-740900.250

NucleoSpin Plasma XS (250)

NucleoSpin Plasma XS (250)

Description

Features 
For rapid purification of circulating DNA from plasma and serum
  •  High recovery even of fragmented DNA > 50 bp
  •  Elution in as little as 5 μL
  •  Concentrated DNA even from dilute samples
  •  DNA ready to use for real-time PCR




Applications*
  •  Circulating DNA from plasma or serum
  •  Ideal for analysis of fetal DNA from maternal plasma
  •  Ideal for detection of tumor markers in plasma

* Kits to be used for research purposes only

Customer Testimonials

"We tested NucleoSpin® Plasma XS using human maternal plasma samples for subsequent fetal blood group genotyping (fetal RHD, RHE, and KEL typing). NucleoSpin® Plasma XS gave a 2.5–3 cycles earlier signal compared to the competitor kit we used so far. I like the easy handling, the short purification procedure, and the good results!"

-Dr. Doescher, DRK Blutspendedienst NSTOB, Oldenburg, Germany

"We tested NucleoSpin® Plasma XS using rat plasma samples and are very satisfied.
In plasma samples from healthy rats, we found significantly higher amounts of DNA (following the High sensitivity protocol) compared to the competitor kit we used so far (PCR target: k-ras). In addition to this, less volume of plasma is sufficient (240 μL in contrast to 1 mL which we needed with the present kit) and the procedure is quicker!"

-Dolores C. García-Olmo, PhD, Unidad de Investigación, Complejo Hospitalario Universitario de Albacete, Albacete, Spain

  

ItemSize Application Price
NucleoSpin Plasma XS (250)

250 Preps

Extraction $1,192.00


Order includes:
NucleoSpin® Plasma XS Columns with Collection Tubes, Collection Tubes (2 mL), buffers, Proteinase K

Principle / Procedure

Rapid purification of circulating DNA from plasma and serum
Increased amounts of circulating DNA in plasma have been found in a variety of disorders including cancer, autoimmune diseases, infections, certain fetal diseases, trauma, stroke, and others. Circulating DNA is known to be highly fragmented and low in concentration which creates difficulties in its purification.
NucleoSpin® Plasma XS is designed for the isolation of fragmented DNA larger than 50 bp from human EDTA blood plasma. The kit is overcoming the limitations of conventional kits (large membrane diameter associated with large default elution volumes and a resulting DNA concentration which is too low to be directly used as PCR template).
NucleoSpin® Plasma XS allows elution in only 5–20 μL, resulting in highly concentrated DNA!

NucleoSpin® Plasma XS procedure 
 

  

New column design – unique advantages


The innovative new thrust ring in a funnel-design allows standard mini prep spin columns to hold a silica membrane of very small diameter.
The small diameter of the NucleoSpin® Plasma XS membrane allows efficient elution in only 5–20 μL resulting in highest DNA concentrations ready for all typical downstream applications.

Increased sensitivity, better results for circulating DNA 

Elution volume depends on membrane diameter!


MACHEREY-NAGEL (MN):
The Xtra Small diameter of the NucleoSpin® Plasma XS membrane allows efficient elution in only 5–20 μL.

Competitor Q (Q):
Even the kit especially designed for very small samples amounts does not have optimized column features for the desired application, thus elution requires at least 20–30 μL.

Get the high DNA concentration you need ... with NucleoSpin® Plasma XS


Application data

Recovery of DNA spikes from plasma
DNA spikes of 50, 100, 150, 250, and 1000 bp were mixed with plasma samples. 
The DNA was subsequently purified with NucleoSpin® Plasma XS and a kit of competitor Q in parallel.



Competitor Q (Q) completely failed to show recovery for fragments smaller than 100 bp and resulted in an overall weaker performance compared to NucleoSpin® Plasma XS (MN) which gave significantly higher recovery rates. 


NucleoSpin® Plasma XS is ideally suited for the detection of fetal DNA in maternal plasma
Starting material: Human maternal plasma used for fetal DNA diagnostics.
Duplex PCR amplification: RHD fragment (Rhesus-gene) and an Amelogenin fragment specific for the male Y-chromosome, amplicons 150–180 bp in size.
Comparison: Roche MagNA Pure Compact Nucleic Isolation Kit I – Large Volume, NucleoSpin® Plasma XS.

Roche MagNA Pure Compact Nucleic Isolation Kit I – Large Volume, 
input 1000 μL plasma, purification according to the user manual, 10 % of the eluate used for PCR (i.e., DNA from approx. 100 μL plasma)
NucleoSpin® Plasma XS, input 240 μL plasma, purification according to the user manual, 40 % of the eluate used for PCR (i.e., DNA from approx. 100 μL plasma)



 
The significantly better performance of NucleoSpin® Plasma XS is demonstrated by an earlier PCR signal of 2.6 cycles (RHD gene) and 2.1 cycles (Amelogenin) respectively.

Data kindly provided by Dr. Doescher, DRK Blutspendedienst NSTOB, Oldenburg, Germany 

NucleoSpin® Plasma XS is superior to competitor Midi kits - Better PCR signals from less volume of plasma
Starting material: Plasma samples originating from rat EDTA blood 
PCR amplification: 165 bp fragment of the k-ras gene (often involved in cancer). 
Comparison: QIAamp DNA Blood Midi, NucleoSpin® Plasma XS

QIAamp DNA Blood Midi, input 1000 μL plasma, purification according to the user manual, modification: 100 μL elution buffer were pre-heated to 56 °C, 5 min incubation on the membrane prior to elution, elution fraction concentrated to 
30 μL, 2 μL of concentrated eluate used for PCR
NucleoSpin® Plasma XS, input 240 μL plasma, purification according to the user manual (High sensitivity protocol), elution with 20 μL, 2 μL of eluate used for PCR




NucleoSpin® Plasma XS shows an earlier PCR signal of 2.1 cycles even though QIAamp DNA Blood Midi represents a 2.7 fold PCR input compared to NucleoSpin® Plasma XS.

Data kindly provided by Dolores C. García-Olmo, PhD, Unidad de Investigación, Complejo Hospitalario Universitario de Albacete, Albacete, Spain 

 

$1,192.00

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