NucleoSpin® Plasma XS Columns with Collection Tubes, Collection Tubes (2 mL), buffers, Proteinase K
Principle / Procedure
Rapid purification of circulating DNA from plasma and serum
Increased amounts of circulating DNA in plasma have been found in a variety of disorders including cancer, autoimmune diseases, infections, certain fetal diseases, trauma, stroke, and others. Circulating DNA is known to be highly fragmented and low in concentration which creates difficulties in its purification.
NucleoSpin® Plasma XS is designed for the isolation of fragmented DNA larger than 50 bp from human EDTA blood plasma. The kit is overcoming the limitations of conventional kits (large membrane diameter associated with large default elution volumes and a resulting DNA concentration which is too low to be directly used as PCR template).
NucleoSpin® Plasma XS allows elution in only 5–20 μL, resulting in highly concentrated DNA!
NucleoSpin® Plasma XS procedure
New column design – unique advantages
The innovative new thrust ring in a funnel-design allows standard mini prep spin columns to hold a silica membrane of very small diameter.
The small diameter of the NucleoSpin® Plasma XS membrane allows efficient elution in only 5–20 μL resulting in highest DNA concentrations ready for all typical downstream applications.
Increased sensitivity, better results for circulating DNA
Elution volume depends on membrane diameter!
The Xtra Small diameter of the NucleoSpin® Plasma XS membrane allows efficient elution in only 5–20 μL.
Competitor Q (Q):
Even the kit especially designed for very small samples amounts does not have optimized column features for the desired application, thus elution requires at least 20–30 μL.
Get the high DNA concentration you need ... with NucleoSpin® Plasma XS
Recovery of DNA spikes from plasma
DNA spikes of 50, 100, 150, 250, and 1000 bp were mixed with plasma samples.
The DNA was subsequently purified with NucleoSpin® Plasma XS and a kit of competitor Q in parallel.
Competitor Q (Q) completely failed to show recovery for fragments smaller than 100 bp and resulted in an overall weaker performance compared to NucleoSpin® Plasma XS (MN) which gave significantly higher recovery rates.
NucleoSpin® Plasma XS is ideally suited for the detection of fetal DNA in maternal plasma
Starting material: Human maternal plasma used for fetal DNA diagnostics.
Duplex PCR amplification: RHD fragment (Rhesus-gene) and an Amelogenin fragment specific for the male Y-chromosome, amplicons 150–180 bp in size.
Comparison: Roche MagNA Pure Compact Nucleic Isolation Kit I – Large Volume, NucleoSpin® Plasma XS.
Roche MagNA Pure Compact Nucleic Isolation Kit I – Large Volume,
input 1000 μL plasma, purification according to the user manual, 10 % of the eluate used for PCR (i.e., DNA from approx. 100 μL plasma)
NucleoSpin® Plasma XS, input 240 μL plasma, purification according to the user manual, 40 % of the eluate used for PCR (i.e., DNA from approx. 100 μL plasma)
The significantly better performance of NucleoSpin® Plasma XS is demonstrated by an earlier PCR signal of 2.6 cycles (RHD gene) and 2.1 cycles (Amelogenin) respectively.
Data kindly provided by Dr. Doescher, DRK Blutspendedienst NSTOB, Oldenburg, Germany
NucleoSpin® Plasma XS is superior to competitor Midi kits - Better PCR signals from less volume of plasma
Starting material: Plasma samples originating from rat EDTA blood
PCR amplification: 165 bp fragment of the k-ras gene (often involved in cancer).
Comparison: QIAamp DNA Blood Midi, NucleoSpin® Plasma XS
QIAamp DNA Blood Midi, input 1000 μL plasma, purification according to the user manual, modification: 100 μL elution buffer were pre-heated to 56 °C, 5 min incubation on the membrane prior to elution, elution fraction concentrated to
30 μL, 2 μL of concentrated eluate used for PCR
NucleoSpin® Plasma XS, input 240 μL plasma, purification according to the user manual (High sensitivity protocol), elution with 20 μL, 2 μL of eluate used for PCR
NucleoSpin® Plasma XS shows an earlier PCR signal of 2.1 cycles even though QIAamp DNA Blood Midi represents a 2.7 fold PCR input compared to NucleoSpin® Plasma XS.
Data kindly provided by Dolores C. García-Olmo, PhD, Unidad de Investigación, Complejo Hospitalario Universitario de Albacete, Albacete, Spain