Catalog No. : MN-740302.48
NucleoSpin DNA Plasma Midi Core Kit
|NucleoSpin DNA Plasma Midi Core Kit||
Principle / Procedure
NucleoSpin® DNA Plasma Midi is designed for the rapid, medium scale preparation of highly pure cell-free DNA from up to 5 mL blood plasma using vacuum filtration. The use of vacuum filtration technology eliminates tedious centrifugation steps and manual handling of columns during the process (e.g., discard of collected column flow through). Using the NucleoVac 96 Vacuum Manifold and the Starter Set Midi up to 24 samples can be processed in parallel. The Starter Set Midi consists of SBS format based column holders enabling the use of both manual vacuum manifolds, such as the NucleoVac 96 Vacuum Manifold as well as manifolds of liquid handling platforms. The set includes Column Holder Midi, Wash Plate Midi, and Elution Tube Holder Midi.
The purification protocol is based on the lysis of the plasma samples using Proteinase K incubation in the presence of a chaotropic salt containing buffer. Following the initial lysis step, a binding buffer is added to the samples and the lysate is loaded onto the binding columns. After loading and vacuum filtration driven binding of the DNA to the silica membrane, impurities are removed by several washing steps. cfDNA is eluted after a final drying step. Typically the processing time of 24 samples is approx. 90 min.
- NucleoVac 96 Vacuum Manifold (740681)
- NucleoVac Vacuum Regulator - for controlling of vacuum (740641)
- Starter Set Midi - for processing NucleoSpin® DNA Plasma Midi under vacuum on NucleoVac 96 Vacuum Manifold or similar manifolds; contains 1 Column Holder Midi, 1 Wash Plate Midi, 1 Elution Tube Holder Midi, and Dummy Columns (740744)