Catalog No. : MN-740780.10
NucleoSpin Soil (10)
- Two alternative lysis buffers and a special additive (Enhancer SX) for optimal processing of various soil samples – high yield and high purity
- NucleoSpin® Bead Tubes with ceramic beads for most efficient cell disruption of Gram-positive and Gram-negative bacteria, archaea, yeast, fungi, and algae in soil, sludge,or sediment
- NucleoSpin® Inhibitor Removal Column to eliminate all PCR inhibitors such as humic substances – DNA is ready-to-use for PCR without dilution
PLoS One 2013 July;8(7):e67699. doi:101371/journal.pone.0067699.
Comparison of DNA Extraction Methods in Analysis of Salivary Bacterial Communities
Lazarevic V, Gaia N, Girard M, Francois P, Schrenzel J. (PUBLICATION)
Find more details in the sections below.
|NucleoSpin Soil (10)||
NucleoSpin® Soil Columns, NucleoSpin® Bead Tubes, NucleoSpin® Inhibitor Removal Columns, Collection Tubes (2 mL), Collection Tubes (2 mL, lid), buffers
- Total DNA from microorganisms in soil and sediment
- Quantify and detect microorganisms from environmental samples
- Typical downstream applications: PCR, real-time PCR, Southern blotting, microarray technology
Principle / Procedure
NucleoSpin® Soil for isolation of total DNA from soil includes two alternative lysis buffers and an additional Enhancer SX, which can be combined with both lysis buffers. The resulting four different lysis systems make the kit suitable for all types of different samples, which is important as soil is very heterogenous in pH and the amounts of organic or anorganic matter like humic substances or polysaccharides. The silica membrane in NucleoSpin® Soil Columns is optimized for quantitative DNA binding. NucleoSpin® Bead Tubes, containing strong ceramic beads, allow a most efficient lysis of microorganisms in the sample. NucleoSpin® Inhibitor Removal Columns are included for convenient elimination of contaminants.
NucleoSpin® Soil allows processing of up to 500 mg (wet weight) starting material. Depending on the individual sample, typical yields are in the range of 2 to 10 μg DNA. The eluted DNA is ready-to-use (undiluted) for subsequent reactions like PCR, restriction analysis, and others.
NucleoSpin® Soil procedure
Alternative lysis systems – select the lysis system most suitable for your samples
A: Upper panel: Total DNA from different starting materials was purified with NucleoSpin® Soil using the two different lysis buffers SL1 and SL2. Both lysis buffers were used with and without Enhancer SX (thus the kit includes four different lysis options in total).
Lower panel: Total DNA from potting soil, river sediment, forest soil, and cropping soil was purified with NucleoSpin® Soil (MN) using the optimal lysis buffer system (see upper pannel) and two competitor kits MP and MO.
B: Tabular overview of yield and purity for all samples shown in Figure A.
NucleoSpin® Soil outperforms competitors in yield and purity!
A: Total DNA from forest soil was purified with NucleoSpin® Soil (MN) and competitor kits (MP and MO).
Compared to the very low yield of competitor MO, the DNA purified with NucleoSpin Soil exhibits a UV-VIS spectrum of pure DNA with a maximum absorption at 260 nm and an ideal A260/A230 ratio of 1.90. DNA purified by competitor MP shows a significant increase in absorption below 260 nm indicating massive copurification of humic substances (A260/A230 ratio of 0.58!) which is additionally shown by its colored eluate (B). Thus, most of the absorption at 260 nm is caused by impurities. DNA quantification based on an A260 measurement highly overestimates the real DNA yield (see DNA on the agarose gel as a comparison).
Complete removal of PCR inhibitors – PCR results even from undiluted eluates!
DNA was purified with NucleoSpin Soil using Lysis Buffer SL1 and Lysis Buffer SL2 with and without Enhancer SX as well as with kits from competitor MP and MO. Then, 2 μL of undiluted eluate were used as PCR template with fungi specific internal transcribed spacer (ITS) primers. Competitor MP failed to yield DNA pure enough to be used undiluted. DNA and inhibitor concentration were both low for competitor MO, but the PCR from river sediment samples was still strongly inhibited. With NucleoSpin® Soil there were at least two conditions for each soil type yielding plenty of DNA and working undiluted in PCR.
Note: Samples failing to show PCR amplification might be rescued by using a diluted portion of the elution fraction as PCR template as the inhibitor will also be diluted. However, diluting the template might also reduce the concentration of a target below its detection limit!
Efficient lysis system – even suitable for difficult-to-lyse microorganisms!
Total DNA from 400 mg cropping soil was purified with NucleoSpin® Soil using Lysis Buffer SL2 in combination with Enhancer SX. 2 μl of undiluted eluates were analyzed in PCR using order specific primer systems.
Lane 1: 1 kbp DNA Ladder (Fermentas)
Lane 2: Procaryotes (16S rRNA gene)
Lane 3: Eucaryotes (ITS)
Lane 4: Fungi (ITS)
Lane 5: Fungi (ß-Tubulin)
Lane 6: Algae, Protozoae, Fungi (18S rRNA)
Lane 7: Gram+ (B. subtilis, cerA)
Lane 8: 1 kbp DNA Ladder (Fermentas)