Catalog No. : NOVA-514161
NEXTflex™ Dual-Indexed DNA Barcodes (97-192)
- Enables deep multiplexing of Illumina DNA libraries for NGS applications
- 8 nt index contained within adapter sequence
- Increase your sequencing scale by pooling up to 192 samples on a single flow cell
- The kits are supplied in a 96-well plate format
- Compatible with Illumina next-generation sequencing platforms
|NEXTflex™ Dual-Indexed DNA Barcodes (97-192)||
|Next Gen Sequencing||$11,007.70|
NEXTflex™ Dual-Indexed Adapter (25 µM)
NEXTflex™ Primer Mix (12.5 µM)
What Sequencing Primers Do I Use for Dual-Indexed Libraries?
Libraries made with the NEXTflex™ Dual-Indexed DNA Barcodes differ from libraries made with the NEXTflex DNA Barcodes in that they have two index sequences, as opposed to a single barcode index. Depending on the sequencing platform and flowcell being used, dual-indexed libraries may require the use of special sequencing primers in place of the primers found in the TruSeq Cluster Kit.
Sequencing of dual-indexed libraries on the MiSeq does not require the use of the TruSeq Dual Index Sequencing Primer Box in addition to the MiSeq Reagent Kit.
Sequencing dual-indexed libraries on the HiSeq or GAIIx, however, may require the use of the TruSeq Dual Index Sequencing Box. The TruSeq Dual Index Sequencing Box, SR (Illumina cat. # FC-121-1003) is required when sequencing dual-indexed libraries on a single-read flow cell. In this case, the Index 2 (i5) Sequencing Primer Mix, HP9, must be loaded in position 16 on the flowcell. If sequencing dual-indexed libraries on a paired-end flow cell for HiSeq or GAIIx, the TruSeq Dual Index Sequencing Box is not required, as the Index 2 primer is grafted on the flow cell.
In summary, sequencing dual-indexed libraries requires additional primers only if sequencing on a single-read flow cell on the HiSeq or GAIIx. Sequencing on a paired-end flow cell on the HiSeq or GAIIx or on the MiSeq does not require additional primers to be purchased in addition to the typical sequencing primer kit for each sequencer.