Catalog No. : 520243

8 microTUBE-50 AFA Fiber H Slit Strip V2 Case (120)

8 microTUBE-50 AFA Fiber H Slit Strip V2 Case (120)

Description

What is a microTUBE-50?

  • 55 μl Volume DNA Shearing

The Covaris microTUBE-50 is engineered to optimize DNA Shearing in a 50 µl sample volume with Focused-ultrasonicators utilizing Adaptive Focused Acoustics (AFA) technology.

  • Optimized for protocols requiring 50 to 55 µl sample volume
  • Accurate, tunable & unbiased fragments
  • High DNA recovery from low sample volumes
  • Isothermal, highly controlled, and repeatable process
  • Optimized for the M220, ME220, S220, E220, and LE220 Focused-ultrasonicators

Optimized Design

microTUBE-50 contains a single AFA Fiber and is optimized for protocols requiring a 50 to 55 µl sample volume. microTUBE-50 utilizes a 8 mm version of the Industry-Standard Covaris microTUBE to eliminate excess head space and the possibility of sample splashing.  2D barcodes provide individual sample tracking.


Designed for batch processing

microTUBE-50 AFA Fiber Plate and 8 microTUBE-50 AFA Fiber Strips are designed for higher throughput applications with the ME220, E220, and LE220 Focused-ultrasonicators.


Highly controllable DNA shearing

DNA shearing with microTUBE-50 is highly reproducible and controllable. Performance is reproducible day to day and sample to sample with standard protocols developed and validated by Covaris.


Note 1: microTUBE-50 AFA Fiber Screw-Cap requires use of an M220, ME220, S220, E220 evolution, E220 and associated holder. Please see “Holders and Racks” below.

Note 2: 8 microTUBE-50 AFA Fiber Strip V2 require use of an ME220, E220 evolution, E220 or LE220 with associated rack.

Note 3: 96 microTUBE-50 AFA Fiber Plate require use of an E220 or LE220.

ItemSize Application Price
8 microTUBE-50 AFA Fiber H Slit Strip V2 Case (120)

Case of 120

Shearing $6,966.00

What is a microTUBE?

The Covaris microTUBE is specifically designed and engineered for compatibility with Focused-ultrasonicators utilizing AFA technology and small sample volumes. The microTUBE is widely used to shear DNA and RNA into fragments with distributions centered from 100 to 1,500 bp (for larger size DNA see our miniTUBE and g-TUBE products). DNA Shearing with AFA and microTUBE is the industry standard and the recommended method for DNA shearing by Illumina, Life Technologies, and Agilent. DNA shearing with Covaris AFA and microTUBE is used by the leading sequencing centers worldwide.

The microTUBE is also ideally suited for other applications with AFA in small volumes such as Chromatin Shearing for ChIP, Tissue disruption and homogenization for biomarker discovery and ADME/Tox screening.

The microTUBE is a critical component of the AFA acoustic circuit, focusing the acoustic energy and optimizing inertial cavitation. microTUBE are made of a special borosilicate glass for low impedance and better transmission of acoustic energy. The integrated AFA fiber helps nucleation of cavitation bubbles and allows more uniform sample processing, which in turn increases the efficiency and reproducibility of nucleic acid shearing. The result is the simultaneous collapse of millions of high energy bubbles that allow Covaris microTUBE to accurately, precisely, and controllably exert mechanical energy on your sample. This energy is able to shear DNA and RNA down to 100 bp one magnitude faster than other technologies. Focused-ultrasonicators utilizing AFA technology is also used extensively to shear chromatin and disrupt tissues.

For higher throughput applications with the E220 and LE220 Focused-ultrasonicators the microTUBE plate, microTUBE racks , and microTUBE Strips are available.




Highly Versatile
DNA samples treated with different AFA treatments and analyzed with Agilent Bioanalyser 12k chip



DNA Concentration independent
DNA samples of different concentration treated with the same AFA treatments and analyzed with Agilent Bioanalyser 12k chip



Highly reproducible across a 96 microTUBEs plate
96 samples processed following a 200bp treatment in a Covaris 96 microTUBEs plate and analyzed with Caliper GX 1k



96 samples processed following a 200bp treatment in a Covaris 96 microTUBEs plate and analyzed with Caliper GX 1k. Peak sizes are plotted for each sample as a function of their position in a row and column.

RNA Shearing



The following table summarizes compatibility between microTUBE and Covaris Focused-ultrasonicator. It also includes which holder to choose. (NC stands for Non Compatible)


$6,966.00

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