Catalog No. : COV-500295
- Proven Covaris AFA shearing process, as used in genome centers worldwide
- Generates tight, highly reproducible fragment distributions
- Optimized shearing programs to generate fragment sizes between 150 and 5,000 bp
- Also effective for low-mass, high epitope recovery chromatin shearing
- Now the “Scientist’s Standard” is in a low-cost, compact, easy to use design
The M220 runs a variety of DNA shearing protocols with Covaris SonoLab 7 software and uses both the Covaris microTUBE™ & miniTUBE™.
Integrated temperature control within the M220 eliminates the need for an external chiller, saving space and cost.
Covaris AFA (Adaptive Focused Acoustics™) transmits acoustic energy at ultrasonic frequencies (>400KHz) and very short wavelengths (~3mm) and is uniquely able to focus the energy into your sample. AFA controls the shearing forces that fragment nucleic acids to the selected size. The process is isothermal, non contact, and quiet.
|Automation Next Gen Sequencing||Please Inquire|
|Regulatory Certifications||CE, ETL Mark (for Product Safety), WEEE|
|Size ||33 (width) 38.7 (depth) 27.3 (height) centimeters|
Ambient temperature: 19°C to 25°C (66°F to 77°C)
Relative humidity: 30% to 70%
|Sample vessels||microTUBE, microTUBE-50, milliTUBE 1 ml and miniTUBE|
|Maximum power||75W PIP (Peak Incident Power)|
|Temperature control||Internal temperature control: Protocol temperature range from 7ºC to 20ºC|
|Applications||DNA shearing 150 bp to 5 kb|
|System Control||Notebook computer interface via USB, operating Microsoft Windows XP SP3 or 7 Professional, with Covaris SonoLab 7 control software installed.|
|Installation / setup||Customer installable|
|Safety||Complies with Low Voltage Directive 2006/95/EC. Certified to IEC/EN/UL 61010-1:2004 “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part1: General Requirements”|
|EMC||Complies with radio frequency emissions and immunity requirements for Class A Industrial/Scientific/Medical (ISM) equipment under EN 61326-1:2005, as well as EN 61000-3-2:2004 and EN61000-3-3:1995. These standards meet the essential requirements of the EU EMC Directive 2004/108/EC. Additionally, the instrument meets FCC Part 15 Class A radio emissions requirements for the USA and ICES-003 Class A for Industry Canada.|
The M220 Focused-ultrasonicator provides the same high-performance shearing results researchers have come to expect from Covaris S-Series & E-Series instruments. As demonstrated below, the M220 Focused-ultrasonicator reproducibly shears DNA from 150bp to 5kb in microTUBEs and miniTUBEs.
Versatile DNA Shearing Performance
Figure above demonstrates M220 DNA shearing performance for fragment lengths from 150bp to 1.5kb in the microTUBE, and to 2, 3 and 5 kbp in the miniTUBE. The M220 provides the same versatile DNA shearing capabilities as the S220, E220, & LE220.
High reproducibility at 300bp DNA Shearing
Figure above displays the reproducibility of the M220 when shearing multiple DNA fragments to a fragment length of 300bp by the same M220. The M220 shearing performance is highly reproducible. DNA was sheared using standard Covaris microTUBEs.
Consistent 3kb Fragment Length DNA Shearing
Figure above demonstrates M220 shearing performance a fragment length of 3kb. Multiple samples were reproducibly sheared to 3kb using Covaris Blue miniTUBEs. The M220 provides consistent DNA shearing performance day-to-day and lab-to-lab.
Tubes and vessels specifically engineered for AFA
microTUBE AFA Fiber Snap-Cap (PN 520045) for 130 µl volume – from 150bp to 1,500bp fragments
microTUBE-50 AFA Fiber Screw-Cap (PN 520166) for 50 µl volume – from 150bp to 800bp fragments
microTUBE-15 AFA Beads Screw-Cap (PN 520145) for 15 µl volume – from 150bp to 550bp fragments
miniTUBE Clear, Blue and Red for 2, 3 and 5 kbp fragments
Note : All microTUBE and milliTUBE require use of an associated holder. Please see “Holders and Inserts” below.
Holders and Inserts for M220 Focused-ultrasonicator
M220 Holder XTU (PN 500414). This holder requires an insert specific to the AFA vessels used.
M220 Holder XTU Insert microTUBE 130 µl (PN 500489). For use with microTUBE AFA Fiber Snap-Cap, truXTRAC FFPE Kits and truChIP Kits.
M220 Holder XTU Insert microTUBE 50 µl (PN 500488). For use with microTUBE-50 AFA Fiber Screw-Cap.
M220 Holder XTU Insert microTUBE 15 µl (PN 500420). For use with microTUBE-15 AFA Beads Screw-Cap.
M220 Holder XTU Insert milliTUBE 1 ml (PN 500422). For use with milliTUBE 1 ml and truChIP Kits.
M220 Holder miniTUBE (PN 500302). This holder is compatible with miniTUBE Clear, Blue and Red
Cleaning and Maintenance
M220 Fill and Drain Accessory Kit
M220 Swab Cleaning Kit