What is a microTUBE?
The Covaris microTUBE is specifically designed and engineered for compatibility with Focused-ultrasonicators utilizing AFA technology and small sample volumes. The microTUBE is widely used to shear DNA and RNA into fragments with distributions centered from 100 to 1,500 bp (for larger size DNA see our miniTUBE and g-TUBE products). DNA Shearing with AFA and microTUBE is the industry standard and the recommended method for DNA shearing by Illumina, Life Technologies, and Agilent. DNA shearing with Covaris AFA and microTUBE is used by the leading sequencing centers worldwide.
The microTUBE is also ideally suited for other applications with AFA in small volumes such as Chromatin Shearing for ChIP, Tissue disruption and homogenization for biomarker discovery and ADME/Tox screening.
The microTUBE is a critical component of the AFA acoustic circuit, focusing the acoustic energy and optimizing inertial cavitation. microTUBE are made of a special borosilicate glass for low impedance and better transmission of acoustic energy. The integrated AFA fiber helps nucleation of cavitation bubbles and allows more uniform sample processing, which in turn increases the efficiency and reproducibility of nucleic acid shearing. The result is the simultaneous collapse of millions of high energy bubbles that allow Covaris microTUBE to accurately, precisely, and controllably exert mechanical energy on your sample. This energy is able to shear DNA and RNA down to 100 bp one magnitude faster than other technologies. Focused-ultrasonicators utilizing AFA technology is also used extensively to shear chromatin and disrupt tissues.
For higher throughput applications with the E220 and LE220 Focused-ultrasonicators the microTUBE plate, microTUBE racks , and microTUBE Strips are available.