Sage HLS Extraction and Library Prep System
Extract. React. Select.
Extract up to 2MB DNA directly from cells
The SageHLS system provides a new approach to DNA extraction. Users load cells suspensions on Sage Science gel cassettes to perform lysis under electrophoretic conditions. This removes degraded and solubilized proteins but leaves intact DNA behind, bound in agarose. A cleavase is then used to carefully fragment the DNA into electrophoretically-mobile sizes. Automated DNA size selection collects the DNA in 6 size bins.
Targeted Purification with CRISPR/Cas9: CATCH*
CRISPR/Cas9 can be used to design targeted purification of large genomic regions or genes. This in vitro application with Cas9, termed CATCH, can enhance study of structural variation and provide valuable phasing information.
*Cas9-Assisted Targeting of CHromosome Segments
Jiang et. al., 2015, Nature Communications, doi:10.1038/ncomm9101
Key Downstream Technologies
- optical mapping
- long read sequencing
- bar-coded linked reads
|Sage HLS Extraction and Library Prep System||
|Automated Electrophoresis Next Gen Sequencing||Please Inquire|
The SageHLS instrument includes a monitor, keyboard, and mouse. A CPU is built in; an external PC is not required.
|Electrophoresis voltage||25V - 100V pulsed field and 150V constant|
|Sample Heating Range||15-60 oC|
|Power requirements||100-240 VAC, 2.5 A, 50-60 Hz|
|Weight||20 lbs / 9 kg|
|Dimensions||12h x 13w x 10d (in) | 31h x 33w x 25d (cm), w/ closed lid|
Kits include 4 or 10 ea. disposable pre-cast gel cassettes. Cassette accommodate 2 samples. Some reagents must be purchased separately.
Workflow Guides- Cassette Kits
Workflow Guides- Cell Suspension Kits