8 microTUBE-15 AFA Beads
What is a microTUBE-15?
Ultra-low Volume DNA Shearing
The Covaris microTUBE-15 is engineered to optimize DNA Shearing in small sample volumes with Focused-ultrasonicators utilizing Adaptive Focused Acoustics (AFA) technology.
- 15 μl sample volume processing
- Accurate, tunable & unbiased fragments
- High DNA recovery from low sample volumes
- Fast, one-step workflow
- Isothermal, highly controlled, and repeatable process
microTUBE-15 incorporates newly developed AFA BeadsTM. Due to their unique surface properties, AFA Beads enable fully controllable and repeatable DNA shearing in very low sample volume and at low acoustic power settings. 2D barcodes on each microTUBE-15 provide individual sample tracking.
Highly controllable DNA shearing
microTUBE-15 DNA shearing is highly reproducible and controllable. Performance is reproducible day to day and sample to sample with standard protocols developed and validated by Covaris.
Note 1: microTUBE-15 requires use of an M220, S220, E220 or LE220 Covaris Focused-ultrasonicator and associated rack or holder. Please see “Holders and Racks” below.
Note 2: Centrifuge microTUBE Adapters are required for all microTUBE-15 AFA Beads Screw-Cap protocols. Please see “Accessories” below.
|8 microTUBE-15 AFA Beads H Slit Strip V2 (12)
Pack of 12
What is a microTUBE?
The Covaris microTUBE is specifically designed and engineered for compatibility with Focused-ultrasonicators utilizing AFA technology and small sample volumes. The microTUBE is widely used to shear DNA and RNA into fragments with distributions centered from 100 to 1,500 bp (for larger size DNA see our miniTUBE and g-TUBE products). DNA Shearing with AFA and microTUBE is the industry standard and the recommended method for DNA shearing by Illumina, Life Technologies, and Agilent. DNA shearing with Covaris AFA and microTUBE is used by the leading sequencing centers worldwide.
The microTUBE is also ideally suited for other applications with AFA in small volumes such as Chromatin Shearing for ChIP, Tissue disruption and homogenization for biomarker discovery and ADME/Tox screening.
The microTUBE is a critical component of the AFA acoustic circuit, focusing the acoustic energy and optimizing inertial cavitation. microTUBE are made of a special borosilicate glass for low impedance and better transmission of acoustic energy. The integrated AFA fiber helps nucleation of cavitation bubbles and allows more uniform sample processing, which in turn increases the efficiency and reproducibility of nucleic acid shearing. The result is the simultaneous collapse of millions of high energy bubbles that allow Covaris microTUBE to accurately, precisely, and controllably exert mechanical energy on your sample. This energy is able to shear DNA and RNA down to 100 bp one magnitude faster than other technologies. Focused-ultrasonicators utilizing AFA technology is also used extensively to shear chromatin and disrupt tissues.
For higher throughput applications with the E220 and LE220 Focused-ultrasonicators the microTUBE plate, microTUBE racks , and microTUBE Strips are available.
DNA samples treated with different AFA treatments and analyzed with Agilent Bioanalyser 12k chip
DNA Concentration independent
DNA samples of different concentration treated with the same AFA treatments and analyzed with Agilent Bioanalyser 12k chip
Highly reproducible across a 96 microTUBEs plate
96 samples processed following a 200bp treatment in a Covaris 96 microTUBEs plate and analyzed with Caliper GX 1k
96 samples processed following a 200bp treatment in a Covaris 96 microTUBEs plate and analyzed with Caliper GX 1k. Peak sizes are plotted for each sample as a function of their position in a row and column.
The following table summarizes compatibility between microTUBE and Covaris Focused-ultrasonicator. It also includes which holder to choose. (NC stands for Non Compatible)