BluePippin Size Selection System
DNA Size Selection for Next-Gen Sequencing, with Pulsed-Field (100bp – 50kb)
Collect HMW DNA with High-Pass Filtering
The BluePippin has the DNA capabilities of the Pippin Prep, plus the extra benefit of pulsed-field electrophoresis for resolving and collecting high molecular weight DNA. For long-range genomic applications, high-pass filtering allows users to collect all fragments above a size threshold set by the user.
Target sizes or ranges of sizes are entered in software, and fractions are collected in buffer. Up to 5 samples/gel cassette may be run, with no possibility of cross-contamination.
Benefits of the BluePippin System
- Saves time and effort compared to manual gel purification
- Sample-to-sample reproducibility streamlines research
- Higher sample quality improves downstream analysis
Key Downstream Technologies
- Mate-pair sequencing
- Single molecule sequencing
- Bar-coded linked reads
|BluePippin Size Selection System||
|Next Gen Sequencing Automated Electrophoresis||Please Inquire|
The BluePippin instrument includes a monitor, keyboard, and mouse. A CPU is built in; an external PC is not required.
Blue Pippin Specs
|Electrophoresis voltage||25V, 100V or 150V constant, 100V pulsed field|
|Optical detection||470 nm excitation, 525 nm emission|
|Power requirements||100-240 VAC, 2.5 A, 50-60 Hz|
|Weight||15 lbs / 7 kg|
|Dimensions||7h x 11w x 21d (in) | 18h x 28w x 53 d (cm)|
Kits include 10 ea. disposable pre-cast gel cassettes. All necessary standards and reagents are included with the purchase.
Pre-Cast Agarose Gel Cassettes for DNA Size Selection
Gel cassettes for DNA selections are available in four agarose concentrations to provide a full range of size selection options between 100bp up to 50kb. For smaller fragment sizes, internal standards may be used for higher accuracy and increased sample capacity (from 4 to 5 samples/cassette). Higher molecular weight fragments require a dedicated lane to run a calibration marker. Run times are as little as 30 minutes, To resolve very large DNA fragments, pulsed-field runs may last several hours.
View the BluePippin cassette reference chart (in Documents section) to explore the DNA size selection options and run times.
Gel Cassette Types and Target Ranges
- 3% agarose, 100-250bp
- 2% agarose, 100-600bp
- 1.5% agarose, 250bp – 1.5kb
- 0.75% agarose, 1-50kb
- miRNA isolation
- DNA sequencing, RNA-seq, genotyping
- DNA sequencing, ChIP-seq
- Mate-pair, single molecule
Sage Pippin: Say Goodbye to Manual Gels
Automated Size Selection of NEBNext Small RNA Libraries with the Sage Pippin Prep
"Size selection of long-chain fragments (8kb) in Nextera Mate Pair library preparation", Dr. Yoshitoshi Ogura and Dr. Yasuhiro Gotoh of Division of Microbiology, Department of Infectious Diseases, School of Medicine, Faculty of Medicine, University of Miyazaki, Japan (Nippon Genetics)
"The effect of size selection of PacBio long-fragment libraries (≧7 kb) (comparison of size distribution and sequencing results)", Dr. Yasuhito Arai of Division of Cancer Genomics, National Cancer Center Institute, Japan (Nippon Genetics)
DNA Control Kit Guides