NucleoSpin Gel and PCR Clean-up (250)
Ex Tax:
$551.00
PCR clean-up and gel extraction - the two-in-one kit with optimized recovery and elution volume

The NucleoSpin® Gel and PCR Clean-up procedure is the easiest way to purify DNA fragments from agarose gels as well as for direct purification of PCR products. The kit includes one buffer for both applications, which contains a pH indicator displaying you the correct pH for optimal kit performance. The purification procedure from enzymatic reactions (e.g., PCR) allows fast and easy removal of enzymes, nucleotides, salts, and other impurities. The NucleoSpin® Gel and PCR Clean-up Columns provide convenient performance for PCR clean-up: After addition of Binding Buffer NTI, the mixture is applied onto the silica membrane. Contaminations are removed by a simple washing step with ethanolic Buffer NT3. For gel extraction, the agarose gel slice is dissolved in high-salt Buffer NT and applied to a NucleoSpin® Gel and PCR Clean-up Column followed by centrifugation and a subsequent washing step. Pure DNA is finally eluted under low ionic strength conditions with slightly alkaline Buffer NE (5 mM Tris/HCl, pH 8.5).

• Two applications in one kit – one buffer with optimal performance for both applications
• High recoveries for fragments down to 50 bp
• Minimized elution volume of 15 μL – highly concentrated DNA
• Binding buffer with pH indicator
• Separate buffers for single-stranded DNA or SDS containing samples available
• Suitable for all gel buffer systems (e.g., TAE, TBE)
• Also available for QIAcube®



• Purification of PCR products
• Extraction of DNA from agarose gels
• DNA extraction from polyacrylamide gels
• Clean-up of single-stranded DNA and RNA extraction from agarose gels (Binding Buffer NTC required, not included in the kit)
• Clean-up of SDS-containing samples (Binding Buffer NTB required, not included in the
   kit, see “Ordering information”)
• Typical downstream applications: cloning, sequencing, PCR, restriction analysis
• NucleoSpin® Gel and PCR Clean-up ensures complete removal of contaminations such as:
- nucleotides, primers
- enzymes
- mineral oil
- PCR additives (e.g., salts, betaine, DMSO)
- detergents (e.g., Tween 20, Triton X-100)
- dyes (e.g., ethidiumbromide, crystal violet, Stain G, Midori Green, Roti®-Safe GelStain, DNA SafeStain)